FIAlab in the Pharmaceutical Industry
SIA
FIAlab’s ProSIAmpler uses a liquid handling technique that automates important processes like sterile sampling, liquid handling, and measurement; this system is the leading bioreactor autosampler on the market. When combined with the SIAlock, 250CC, and SIAsoft, this system offers a multitude of benefits for laboratory operations. The ProSIAmpler, with its variable sample volume, draws ranging from 25uL to 10,000uL, providing flexibility and precision in sample handling. It can efficiently draw and analyze bioreactor samples, saving time and increasing automation.
The ProSIAmpler can monitor bioreactors' nutrients, pH, cell densities, and viabilities, in-situ, using ion-selective electrodes (ISE). This unlocks the potentiometric determination of several bioreactor parameters that are essential for optimal conditions where the ProSIAmpler can be programmed to automatically sample upon certain analyte concentrations or pH levels.
Advantages
Application Range
Suited to run a broad range of applications.
On-line Monitoring
Real-time monitoring and process control.
Python Scripting
Build custom protocols in SIAsoft.
Automation
Sample prep to LCMS injection in one software.
Plastics
No pipette tips or 96-well plates.
Reagent Waste
Little reagent waste and low consumable costs.
SIA Products
ProSIALH
FIAlab’s ProSIALH is a sampling and pretreatment solution that automates bioreactor peptide or glycan monitoring for the biopharmaceutical community. This technology helps reduce overheads, saves time, and cuts plastic usage. The ProSIALH does automated titer calculation and automatically adjusts the amount of sample pushed onto the purification column to match what the assay requires.
ProSIAmpler
FIAlab’s ProSIAmpler can monitor bioreactors' nutrients, pH, cell densities, and viabilities, in-situ, using ion-selective electrodes (ISE). This unlocks the potentiometric determination of several bioreactor parameters that are essential for optimal conditions where the ProSIAmpler can be programmed to automatically sample upon certain analyte concentrations or pH levels
Sample Pretreatment Assays
This automated in-line LC peptide mapping pre-treatment method can be used on any LC unit setup for the multi-attribute monitoring (MAM) of biotherapeutics. The method utilizes a denaturant, reductant, alkylation agent, desalting, and trypsin digestion to prepare a biological sample prior to LCMS analysis.
Peptide Mapping
Peptide Mapping Publication:
This method is for the determination of amino acid species in cell culture media. The method utilizes the Water’s proprietary labeling reagent “AccQ Tag” for derivatizing amino acids prior to HPLC analysis. Intended as a single reagent chemistry, the Amino Acid Analysis package is used for cell culture monitoring.
Amino Acid Monitoring
Amino Acid Publication:
The glycan system automates sample handling to determine stoichiometric relationships between different sugar moieties on monoclonal antibodies (mAbs). This in-line process handles fluid samples by some or all of the following operations: Pro A purification, PNGase enzymatic digestion, fluorescent labeling, and enrichment in porous graphitic carbon (PGC) column.
Glycan Analysis
Glycan Publications: