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FIALab Fluidics Intelligently Automated
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Cl-C-1-1 Chloride · in-line dilution · live flow schematic Mercuric-thiocyanate chloride assay with in-line dilution: sample + diluent pass the DC coil before loading the loop. NaCl carrier; Fe(SCN)²⁺ colour read at 520 nm. REAGENT GRID · add / remove rows Carrier R1 R2 Diluent Sample PUMP Speed 20% C · NaCl carrier → valve R1 · HNO₃ + Brij → M1 R2 · Hg(SCN)₂ – Fe(NO₃)₃ → M2 D · diluent → DC S · sample → DC → valve DCdilution coil loop 35 µL INJECTION VALVE 6-port rotary M1 M2 RC1 RC2 RC3 RC4 DETECTOR Spectrometer λ₁ = 520 nm λREF = 700 nm Flow cell · 2.5 mm path W W carrier in to system sample in waste METHOD — Cl-C-1-1 · chloride by mercuric thiocyanate · in-line dilution (2.5 mm cell) Stream colours Carrier (NaCl) Sample R1 HNO₃ + Brij Mixed slug R2 Hg(SCN)₂ – Fe(NO₃)₃ Diluent (in-line dilution via DC) Injection valve · auto-cycle 6-port rotary · loop 35 µL · diluted sample loads the loop INJECT 3 s every 15 s · slug ~7 s to cell Loop flush: samplemixedcarrier Pump tubing (ID · collar) S 1.02 mm White/White · D 1.85 mm Green/Green C 1.14 mm Red/Red · R1, R2 1.42 mm Yellow/Yellow Pump 20% · cell 2.5 mm · 520/700 nm LOAD · filling the sample loop