The SIChrom™ Accelerated Liquid Chromatograph System
Onyx™ Advantages & Benefits: Monolithic vs. Particle-Based Structure

Until recently, HPLC columns have been made of particle-based materials, usually silica. By their very nature, small particles, when packed tightly into an HPLC column, create a significant resistance to the flow of the solvent/sample mixture along with other limitations highlighted below.

Traditional Silica "Particle-Based" Column

• Individual silica particles
• High flow resistance:
  Limits ability to shorten run times
• High backpressure:
  Reduces life of pumps, seals, and column
• Reduced throughput:
  Long run times
• Bed splitting and voiding possible:
  Shortens column life and affects
  reproducibility, lowers efficiency

 Onyx™ “Monolithic” Column

• Monolithic porous silica rod
• High flow rates:
  Due to high porosity
• Low backpressures:
  Less stress on system and column
• Increased throughput:
  Significantly shorter run times
• No inlet bed settling:
  Increased reliability, reproducibility, and lifetime, maintains efficiency

Note:  FIAlab Instruments is not affiliated with Phenomenex.
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