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Reagents:
Carrier:
Milli-Q water spiked with 0.5% (v/v)
ammonia free seawater or 0.2 g/l NaCl
Reagent 1: OPA Reagent
*
Borate buffer solution: 30 g/l of
disodium tetraborate decahydrate. The
solution was slightly turbid and was
filtered using a Whatman GF/C membrane.
It remains stable for several months in
a polyethylene vial.
*
OPA
solution: 2 g of standard grade
ophtaldialdehyde (P- 1378 Sigma
Chemical) in 50 ml of ethanol (Carlo
Erba 414607). Dissolution, performed in
the dark by shaking, took a few minutes.
This solution can be stored refrigerated
in a glass bottle for at least 2 months.
*
Sulfite solution: 8 g/l of sodium
sulfite. The solution was found to be
stable for at least 2 months at room
temperature in a glass bottle.
The working reagent Working reagent
(WR). In a borosilicate bottle, 500 ml
of borate buffer were introduced and the
following reagents added, with mixing
after each addition: 10 ml of OPA
solution, 1 ml of sodium sulfite
solution and 0.1 ml of a 30% Brij
solution. This reagent, rapidly
protected from light, was left to stand
several hours. It can be stored in
the dark at room temperature for at
least 2 months.
Reagent 2: Not used
Plug R2 port with
Teflon Plug
Standards:
100ml ICAMU-100 (ammonia
standard)
Source: 727-524-7732 -
www.exaxol.com
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