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FIAlab standard method for dissolved
Iron (FE) assay using the
FIAlab-2500/2600/2700 system.
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Assay |
Typical Throughput |
Concentration Range |
Notes |
|
Iron (Mid) |
140 samples/hour |
0.025 to 100 ppm |
1 cm flowcell |
|
Iron (Low) |
70 samples/hour |
0.0025 to 10 ppm |
10 cm flowcell |
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Iron (Ultra Low) |
45 samples/hour |
0.0005 to 0.2 ppm |
50 cm flowcell |
Principle:
1,10-phenanthroline
chelation chemistry to Fe2+. This
Fe-complex has an absorbance maximum around
510nm.
Comments:
A heater is not necessary.
The flowrate of the pump should be set
to 55 for Iron. The FIA LOV connections
B should be bridged by a simple tubing.
For typical Iron assays (.5 to 100 ppm),
make the sample loop from three inches
of .03” ID tubing.
For ultra low concentrations use a
36" sample loop.
For the low end of these ranges,
care must be taken to use clean
glassware and extended washouts of
tubing
on the system to prevent
crossover contamination.
Recommended wavelengths 510 primary and
650 reference
For seawater based assays, standards
must match the salinity of the samples
as close as possible.
Alternative method:
A common alternative Iron method is
based on Ferrozine,
For use with Iron (II) and total dissolved iron,
especially for ultra low level (< 100
nmol/l), consider using the method
described in this paper: “COLORIMETRIC
FLOW-INJECTION ANALYSIS OF DISSOLVED
IRON IN HIGH DOC WATERS”, MICHAEL J.
PULLIN1 and STEPHEN E. CABANISS*,
Department of Chemistry and Water
Resources Research Institute,
Kent State University,
Kent,
OH
44242,
USA.
Interferences:
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Reagents:
Carrier: DI Water
1-Liter DI Water with surfactant.
4
drops of dishwashing liquid, or Brij per
500
ml
Reagent 1:
1,10-phenanthroline chelation chemistry
AQUANAL-plus reagent kit for iron
analysis is used and is based on
1,10-phenanthroline chelation chemistry
to Fe2+.
(Source -
product number
37404 or 37444 at
http://www.sigmaaldrich.com)
Dilute
reagent by mixing one drop per one ml of
DI water.
Reagent 2: Not used
Plug R2 port with Teflon Plug
Standards:
500ml I-4784-500 (Iron standard)
Source: 727-524-7732 -
www.exaxol.com
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Please email or phone FIAlab Instruments
for additional product information.
Email:
fialab@flowinjection.com,
Voice: 425-376-0450 or 1-800-963-1101,
Fax: 425-376-0451
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