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Chloride  

FIAlab standard method for chloride assay using the FIAlab-2500/2600/2700 system.
Assay Typical Throughput Concentration Range Notes
Chloride (Mid) 120 samples/hour 1 to 50  mg Cl-/L 1 cm flowcell
Chloride (Low) 60 samples/hour 0.1 to 5  mg Cl-/L 10 cm flowcell

 

 Principle:

 The chloride assay is based on the liberation of the thiocyanate ion (SCN) from mercuric thiocyanate, through sequestration of mercury by the chloride ion, to form un-ionized, but soluble mercuric chloride. The liberated SCN then reacts with the ferric ion to form highly colored ferric thiocyanate.

 

 Comments:

A heater is not necessary.   The flowrate of the pump should be set to 55 for Chloride.  The FIA LOV connections B should be bridged by a simple tubing.   Recommended wavelengths 480 primary and 650 reference

The sample loop should be three inches of .03” ID tubing.

Interferences:

Components which reduce iron (III) to iron (II) and mercury (III) to mercury (II),  e.g. sulfite, thiosulfate.

Reagents: 

Carrier: DI Water
1-Liter DI Water.  Matrix matching is recommended, especially for low concentration samples.

 Reagent 1:  Mix equal parts (15 ml each) of Mercuric Thiocyanate Stock solution (Component A) and Ferric Nitrate Stock Solution (Component B)  diluted to 100ml with reagent water.

 Component A:  Mercuric Thiocyanate Stock solution: Mercuric Thiocyanate in Methanol           
Component B: Ferric Nitrate Stock Solution: Ferric Nitrate in Nitric Acid diluted with reagent water 
Note: If bubbles become an issue, sticking in the flowcell and other parts of the manifold, then add 1-gram Brij-35 (detergent) per liter to Reagent 1.

 Reagent 2: Not used

Plug R2 port with Teflon Plug  

 
 Standards:
100ml ICCHL-100 (Chloride standard)
 Source: 727-524-7732 - www.exaxol.com   

 
Please email or phone FIAlab Instruments for additional product information.
 Email: fialab@flowinjection.com, Voice: 425-376-0450 or 1-800-963-1101,  Fax: 425-376-0451