FIAlab Instruments, Inc.
14450 NE 29th Place STE 113
Bellevue, WA 98007 USA
Voice: 425-376-0450
Fax: 425-376-0451
fialab@flowinjection.com
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FIAlab standard method for chloride
assay using the
FIAlab-2500/2600/2700 system.
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Assay |
Typical Throughput |
Concentration Range |
Notes |
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Chloride (Mid) |
120 samples/hour |
1 to 50
mg Cl-/L |
1 cm flowcell |
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Chloride (Low) |
60 samples/hour |
0.1 to 5
mg Cl-/L |
10 cm flowcell |
Principle:
The chloride assay is based on the liberation of
the thiocyanate ion (SCN) from mercuric
thiocyanate, through sequestration of mercury by
the chloride ion, to form un-ionized, but
soluble mercuric chloride. The liberated SCN
then reacts with the ferric ion to form highly
colored ferric thiocyanate.
Comments:
A heater is not necessary.
The flowrate of the pump should be set
to 55 for Chloride.
The FIA LOV connections B should be bridged by a simple tubing.
Recommended wavelengths 480 primary and
650 reference
The
sample loop should be three inches of
.03” ID
tubing.
Interferences:
Components
which reduce iron (III) to iron (II) and
mercury (III) to mercury (II), e.g.
sulfite, thiosulfate.
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Reagents:
Carrier: DI Water
1-Liter DI Water. Matrix matching is
recommended, especially for low
concentration samples.
Reagent 1:
Hg(SCN)2+Fe(III)
Labchem's Color Reagent Cat No
LC13260.
Alternative source - Chloride Color
Reagent (Technicon No. T01-0352).
Additional information can be found at:
http://www.epa.gov/glnpo/lmmb/methods/methd140.pdf
Note: If bubbles become an issue,
sticking in the flowcell and other parts
of the manifold, then add
1-gram Brij-35
(detergent) per liter to Reagent 1.
Reagent 2: Not used
Plug R2 port with
Teflon Plug
Standards:
100ml
ICCHL-100
(Chloride standard)
Source:
727-524-7732
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www.exaxol.com
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Please email or phone FIAlab Instruments
for additional product information.
Email:
fialab@flowinjection.com,
Voice: 425-376-0450 or 1-800-963-1101,
Fax: 425-376-0451
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