FIAlab Instruments
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PMT-FL Flow Thru Fluorometer

For Low Level Fluorescence and Chemiluminescence Assays
 

PMT-FL shown with optional S2-Mini peristaltic pump

 

PMT-FL with sensor drawer slid open

 

PMT-FL connected with MicroSIA System

 

Specifications
 

·        Photon counting PMT detector

·       Dimensions: ~5 X 7 X 10 in (~13 X 18 X 25.4 cm)

·        310nm - 750nm spectral range

·        Pulse pair resolution: 10ns

·        Responsivity @ 400nm: 5 X 1017 cps/Watt

·        PMT dynamic range:  2.0E06 

·        10 - 100 microliter flow through cuvette (please inquire for size options)

·        RS232 Control (also available with USB interface)

·        Includes FIAlab for Windows operating software

·        Can be interfaced through Visual Basic, VC++, Labview, HPVEE, and other environments

·        Filters: each PMT-FL order includes your waveband choice of both excitation and emission filters (please enquire). 

Example  Assays Applications for the PMT-FL

 Fluorometric ammonium analysis:  ultra low level assays, parts per billion sensitivity.


 F4551: Fluorescein di(b-D-glucuronide),Hydrolyzes to fluorescent fluorescein. Has been used to detect ß glucuronidase activity, assay for lysosomal enzyme release from neutrophils

 

L9009: Luciferase Photinus pyralis. The bioluminescent reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light.  Luciferase can be used to detect trace amounts of ATP.

 

GUS-A: b-Glucuronidase Fluorescent Activity Detection Kit. The substrate used in this kit is MU-GlcA (4-methylumbelliferyl b-D-glucuronide), a widely used fluorogenic substrate for determination of glucuronidase activity.

The PMT-FL is a highly sensitive photomultiplier based flow through detection system ideally suited for ultra low fluorescence, chemiluminescence or bioluminescence measurements.  Parts per trillion sensitivity.  Heavy-duty chemical resistant housing built to withstand industrial environments.  Besides flow through capability, the system also accepts standard 1-cm path length cuvettes for manual measurements.

 

The PMT-FL is built to order with either an internal excitation lamp or an SMA terminated fiber optic cable for use with an external lamp (see configuration options listed below).  Emission and excitation filters are mounted in user accessible slots inside the enclosure, allowing for quick removal for exchange.

 

The PMT-FL plugs into the RS-232 serial port of a personal computer. Included with the system is Windows based software, allowing the user to set the integration time and voltage counts, and to obtain time histories of the measurements, both plotted and tabulated.  The software can also automatically create calibration curves, as well as controlling a host of additional devices such as FIAlab's FIA/SIA systems, syringe and peristaltic pumps, injection valves, selection valves, and autosamplers (please inquire for details).  For users who desire to control the PMT-FL from their own software project, included is an ActiveX control for use with Visual Basic, VC++, Labview, or nearly any other ActiveX compatible development environment.

 

Configuration Options

 

Optional internal excitation sources include:

  Laser Diodes (532 nm, 635 nm, 650 nm)

  Quartz tungsten lamp (440 - 750 nm)

  Mercury Vapor lamps (350 - 400 nm)

  UV LED lamp (270 - 410 nm)

  Various visible LED lamps

 

Optional fiber optic excitation sources include:

   All above + Deuterium lamp (200 - 400 nm)

 

Flow through Heater:  

Heated flowcell, ambient to 55 C, +/- 0.5 C accuracy.

 Please inquire about our new dual channel PMT-FL system (below).  Measure two different emission wavelength bands simultaneously/independently from one sample.

 

 Please inquire about our new PMT-FL-NIR option (below) for long wavelength emission sensitivities out to 860 nm

 

 

Limits of Detection
 

10 parts per trillion  - measured with Fluorescein @ 250 msec integration and internal tungsten lamp
 

27 parts per trillion - measured with 4 MU @ 250 msec integration time and internal mercury vapor lamp
 
Please email or phone FIAlab Instruments for additional product information.
 Email: fialab@flowinjection.com, Voice: 425-376-0450 or 1-800-963-1101,  Fax: 425-376-0451
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