FIAlab Instruments
 Leaders in Flow Injection Technology

FIAlab’s MicroSIA system is the smallest complete Sequential Injection Analysis system available.  Measuring only 5 inches wide, 6 inches high, 6 inches deep, and weighing only around 8 pounds, this highly portable unit is ideal for the laboratory where bench space is at a premium.      Multiple MicroSIA units are easily interconnected for simultaneous multichannel measurements.   This is the recommended system for on-line applications, where low sample and reagent usage and low waste production is critical.  The MicroSIA system allows the user to change the mixing, dilution, reagent/sample ratios, stop time, monitored wavelengths, integration time, and many other assay parameters through software settings.  No need to swap out expensive manifolds every time a new type of assay is required.  The MicroSIA system is operated by FIAlab for Windows control software.

Three Channel System used for Simultaneous Measurements of Three Analytes

Sequential Injection for the Assay of Biomolecules, including Enzymatic Assays, Bioligand Interaction Assays and Chromatography of Biomolecules are usually carried out on different instruments, which necessitates mastering various types of hardware and software.  This fragmented approach supports a view of an apparent incompatibility of these techniques, thus obscuring similarities of their underlying biochemical reactions and kinetics. It is the versatility of programmable flow combined with a UV/Vis (or fluorescence) detector that allows the gap between these techniques to be bridged by a single MicroSIA with FIAlab’s unique Lab-On-Valve manifold.

SIA-performed Bioligand Interaction Assays (BIA) are based on the monitoring of UV/VIS spectra of translucent beads (Sephadex, Sepharose) during their interaction with biomolecules in assayed solution. The protocol starts by injecting microliter volumes of beads into the flow cell, where the beads are captured and monitored while the analyte solution is passed through.   See MPG Movie Clip demonstrating the ease at which microbeads are manipulated from the holding port to the optical flowcell, all within the LOV manifold.  (4 MB long, may take a minute or so to download).

Sequential Injection Affinity Chromatography is a redesigned variant of conventional affinity chromatography.  The initial design of Sequential Injection Chromatography (SIC) by Satinsky et. al.,  used programmable flow and the Lab-On-Valve  platform to carry out assays, and small scale separation of biomolecules on columns filled with Sephadex or Sepharose.

SIA-performed Enzymatic Assays are based on reaction rate measurements, carried out in a stopped-flow mode, while the reaction mixture is held for monitoring within the flow through cell.  Substrate assays include glucose, lactate, urea, glycerol, ethanol.

MicroSIA System with Secondary Syringe Pump	Close-up of Lab-On-Valve Manifold	The MicroSIA is also available with a standard Multiposition Valve, as shown above with the PMT-FL

The MicroSIA system can be used for online measuring many common analytes, including: Ammonia, Nitrite, Nitrate/Nitrite, Phosphate, Chloride, Silicate, Boron, Chromium, Copper, Iron, Zinc, Bromine, Chlorine, Iodine, Sulfide,  Sulfate, Fluoride,  Hydrogen Peroxide, Iron,  Manganese,  Molybdenum, pH, and Nickel.  Many others are possible.  Low cost standardized reagent test kits for these assays  are commercially available.  Please inquire concerning compatibilities with your specific requirements.

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