The advantage of Sequential Injection is that it consumes  only around 20 microliters of sample and around the same amount of reagent for an assay. This makes this instrument  compatible with many low throughput sampling probes (such as the 19mm Flownamics Instruments in situ probe). Commercially available  reagent kits are routinely used for  ammonia, glucose, glycerol and iron with SIA protocols applied for  "at line" and "on line" fermentation monitoring   The recommended system for fermentation monitoring is the MicroSIA system.

Since the FIAlab SIA/FIA systems are compact,  they can be easily accommodated close to the  fermentor.  This allows continuous monitoring during the fermentation process, while the MicroSIA system is periodically activated and  periodically recalibrated by using a standard analyte solution.

Reference:  "Micro sequential injection: Fermentation monitoring of ammonia, glycerol, glucose, and free iron using the novel lab-on-valve system", Chao-Hsiang Wu, L. Scampavia, J. Ruzicka, B. Zamost, The Analyst, February Issue 2001 (click to view, ~ 835 Kb, pdf format).

MicroSIA System Configured for Sequential Injection Ion Exchange Chromatography