FIAlab Instruments
 Leaders in Flow Injection Technology

Sequential Injection for the Assay of Biomolecules, including Enzymatic Assays, Bioligand Interaction Assays and Chromatography of Biomolecules are usually carried out on different instruments, which necessitates mastering various types of hardware and software.  This fragmented approach supports a view of an apparent incompatibility of these techniques, thus obscuring similarities of their underlying biochemical reactions and kinetics. It is the versatility of programmable flow combined with a UV/Vis (or fluorescence) detector that allows the gap between these techniques to be bridged by a single MicroSIA or FIAlab-3500 system with FIAlab’s unique Lab-On-Valve manifold.

MicroSIA System Configured for Sequential Injection Ion Exchange Chromatography

SIA-performed Enzymatic Assays are based on reaction rate measurements, carried out in a stopped-flow mode, while the reaction mixture is held for monitoring within the flow through cell.  Substrate assays include glucose, lactate, urea, glycerol, ethanol.

Sequential Injection Affinity Chromatography is a redesigned variant of conventional affinity chromatography.  The initial design of Sequential Injection Chromatography (SIC) by Satinsky et. al.,  used programmable flow and the Lab-On-Valve  platform to carry out assays, and small scale separation of biomolecules on columns filled with Sephadex or Sepharose. 

 
SIA-performed Bioligand Interaction Assays (BIA) are based on the monitoring of UV/VIS spectra of translucent beads (Sephadex, Sepharose) during their interaction with biomolecules in assayed solution. The protocol starts by injecting microliter volumes of beads into the flow cell, where the beads are captured and monitored while the analyte solution is passed through.   Native biomolecules typically absorb at 260 and 280 nm while labeled molecules absorb visible light (and some also emit fluorescence). Thus both nonlabeled and labeled biomolecules are detected simultaneously. 

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