SIA-performed Enzymatic Assays are based on reaction rate measurements, carried out in a stopped-flow mode, while the reaction mixture is held for monitoring within the flow through cell.  Substrate assays include glucose, lactate, urea, glycerol, ethanol.

Sequential Injection Affinity Chromatography is a redesigned variant of conventional affinity chromatography.  The initial design of Sequential Injection Chromatography (SIC) by Satinsky et. al.,  used programmable flow and the Lab-On-Valve  platform to carry out assays, and small scale separation of biomolecules on columns filled with Sephadex or Sepharose. 

 
SIA-performed Bioligand Interaction Assays (BIA) are based on the monitoring of UV/VIS spectra of translucent beads (Sephadex, Sepharose) during their interaction with biomolecules in assayed solution. The protocol starts by injecting microliter volumes of beads into the flow cell, where the beads are captured and monitored while the analyte solution is passed through.   Native biomolecules typically absorb at 260 and 280 nm while labeled molecules absorb visible light (and some also emit fluorescence). Thus both nonlabeled and labeled biomolecules are detected simultaneously.