Plug and Play
All FIAlab instruments can be ordered fully configured with a laptop PC loaded with software, and tested before shipping.
Sequential Injection
SI uses computer control for optimization of all steps of an assay protocol. The result is high reproducibility, low reagent consumption and minimized waste generation. The versatility and robustness of the SI technique makes it a preferred choice for laboratory assays, and for monitoring of industrial and environmental processes. SI is compatible with virtually all detectors or sensors, including spectroscopic and electrochemical.
Sequential Injection is based on injection of a sample into a carrier solution, moving in a preprogrammed way. Sample and reagent solutions are selected by means of a mulltiposition valve and their volumes, on a microlitre scale and controlled by a syringe pump. Mixing is achieved by flow reversals, and reaction rates may be monitored, while the reacting mixture is held within a flow cell. The new
generation of this technique, the microSequential Injection (mSI), miniaturized by Lab-on-Valve® technology represents a quantum leap in performing reagent-based assays in the laboratory and in the field. For further details see page on SI- Principles and the Tutorial.
microSequential Injection in
Lab-on-valve configuration
micro Sequential Injection
The microSIA LOV system offers unique capabilities. It is not only versatile, but it is also the smallest automated analyzer available on the market. With a footprint of 12x14cm, and only 14cm high and weighing only 3.5kg, this portable unit is ideal for in situ applications and for the laboratory where bench space is at a premium. It uses microlitre volumes of samples and reagents and generates 50 times less waste per assay than any other flow based automated analyzer. Its versatility makes it an ideal research tool, and its low reagent consumption makes it the most economic system for serial assays. Robustness and reliability make microSI well suited for on line applications. For the first time, an assay developed and tested in a laboratory can be transferred in the field or in a process using the same instrument.
microSIA with LOV module
optical bench and stand
FIAlab 3200 with LOV module
Dual Pump micro Sequential System
The FIAlab-3200 LOV Dual Syringe SI instrument is designed for research and development projects. Two independently controllable syringe pumps are suited for assays based on gas diffusion, or for gradient generation used for automated titrations, or for separation of analytes from matrix by means of sorbent extraction. An auxiliary peristaltic pump can be incorporated upon request.
Advantages of dual syringe system. Syringe pumps can be controlled simultaneously and independently with regards to direction, flow rate, and volume. Programmable gradients can thus be created.- The system provides a wide range of flow rates. By simultaneously using a small and a large syringe, one can significantly increase the system's flow rate dynamic range. - The dual syringe combination is ideal for auto diluting high concentration samples prior to analyses. The system can be run with seamless uninterrupted flow; while one syringe is refilling, the other is emptying.
Multichannel microSI
Multichannel SI systems are best assembled by daisy chaining several microSI single channel instruments. The advantage of this approach is versatility, since the instrument can be easily reconfigured for different sets of analytes, improved reliability – if one channel fails, the other channels remain unaffected.
The LOV platform integrates manifold components (valve, microcolumns and flow cell), within a transparent monolithic structure. This unique technology transforms the SI technique into robust microfluidic format, where sample and reagent consumption is reduced to microliter volumes. The user can observe individual steps of an assay protocol and thus efficiently optimize all individual operations with an unprecedented insight.
For further details see page SI-Lab-on-valve.
Lab-on-Valve®
6 position LOV module
fabricated from Perspex®
Lab-on-Valve® Flow Cell Configuration for Spectrophotometry,
Fluorescence and Chemiluminescence
Spectrophotometry
Fluorescence
Chemiluminescence
The quartz optical fibers that connect the flow cell within the LOV module to light source and detector can be easily configured to monitor :
1) UV-VIS spectrophotometry by the mounting the optical fibers axially with light path .
2) Fluorescence by mounting the optical fiber at the right angle (as shown).
3) Chemiluminescence by mounting the fibers at right angles (as shown), in order to maximize the monitored volume.
For further details see page SI Lab-on-valve.
Recommended Instrument Configurations
Typical Configurations and Accessories
include light sources, detectors, and
For further information on principles
consult Sequential Injection page.
8 position LOV fabricated from
solvent resistant ULTEM
Sequential and Flow Injection System
The FIAlab-3500 system is our most flexible instrument. It performs analyses in both SIA and FIA format. With a multiposition valve, a high resolution microsyringe pump and a 4 channel peristaltic pump, this unit is also suitable for exploration of chemical reactions, low pressure column separations, dialysis, and gas diffusion.
The FIAlab-3500 system is ideal for developing new FIA/SIA methods and testing new sensor technologies.
FIAlab 3500 with Spectrophotometer and Tungsten lamp.
The Fialab-3500 system optionally includes multiple RS-232 ports, A/D data collection channels, D/A outputs, TTL outputs, and 24 VDC relays. Peripheral compatible analytical instruments can interface directly with the FIAlab-3500 simply by plugging into the back panel and include autosamplers, additional syringe pumps, additional peristaltic pumps, external selector and injector valves, and the PMT-FL. The instrument is compatible with UV-VIS spectrophotometry, fluorescence, chemiluminescence and electrochemical detectors which are integrated with the instrument control by means of FIAlab software.
